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phosphospecific antibodies against stat2  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology phosphospecific antibodies against stat2
    Fig. 1. PIV5 V protein binds human but not mouse <t>STAT2.</t> Mouse NIH3T3 cells or human 2fTGH and HEK293Tcells were transfected with either FLAG-tagged PIV5 V protein (V) or FLAG vector control (C). Parallel samples of NIH3T3 were co-transfected with human STAT2 expression vector (hST2). Whole cell extracts were immunoprecipitated with FLAG-M2 agarose beads, eluted with SDS–PAGE buffer and processed for immunoblot with specific antibodies indicated.
    Phosphospecific Antibodies Against Stat2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 558 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphospecific antibodies against stat2/product/Santa Cruz Biotechnology
    Average 95 stars, based on 558 article reviews
    phosphospecific antibodies against stat2 - by Bioz Stars, 2026-02
    95/100 stars

    Images

    1) Product Images from "Enabled interferon signaling evasion in an immune-competent transgenic mouse model of parainfluenza virus 5 infection."

    Article Title: Enabled interferon signaling evasion in an immune-competent transgenic mouse model of parainfluenza virus 5 infection.

    Journal: Virology

    doi: 10.1016/j.virol.2007.10.001

    Fig. 1. PIV5 V protein binds human but not mouse STAT2. Mouse NIH3T3 cells or human 2fTGH and HEK293Tcells were transfected with either FLAG-tagged PIV5 V protein (V) or FLAG vector control (C). Parallel samples of NIH3T3 were co-transfected with human STAT2 expression vector (hST2). Whole cell extracts were immunoprecipitated with FLAG-M2 agarose beads, eluted with SDS–PAGE buffer and processed for immunoblot with specific antibodies indicated.
    Figure Legend Snippet: Fig. 1. PIV5 V protein binds human but not mouse STAT2. Mouse NIH3T3 cells or human 2fTGH and HEK293Tcells were transfected with either FLAG-tagged PIV5 V protein (V) or FLAG vector control (C). Parallel samples of NIH3T3 were co-transfected with human STAT2 expression vector (hST2). Whole cell extracts were immunoprecipitated with FLAG-M2 agarose beads, eluted with SDS–PAGE buffer and processed for immunoblot with specific antibodies indicated.

    Techniques Used: Transfection, Plasmid Preparation, Control, Expressing, Immunoprecipitation, SDS Page, Western Blot

    Fig. 2. Expression of human STAT2 in transgenic mice. (A) The 2555-bp open reading frame encoding human STAT2 was flanked by the human ubiquitin C promoter on the 5′ end and an SV40 splice site and poly A tail on the 3′ end. (B) NIH3T3 cells were transfected with the 5xISRE-luciferase plasmid with or without the hSTAT2 transgene construct, infected with PIV5 for 24 h prior to luciferase assay. Normalized to co-transfected Renilla luciferase. Bars indicate the mean normalized to percent of maximum (n=3) and error bars indicate standard deviation. (C) Ubiquitous STAT2 expression in transgenic mice. Protein extracts (20 μg) from isolated transgenic mice were subject to immunoblot to detect human STAT2. Ubiquitous expression of the transgene throughout the mouse was observed.
    Figure Legend Snippet: Fig. 2. Expression of human STAT2 in transgenic mice. (A) The 2555-bp open reading frame encoding human STAT2 was flanked by the human ubiquitin C promoter on the 5′ end and an SV40 splice site and poly A tail on the 3′ end. (B) NIH3T3 cells were transfected with the 5xISRE-luciferase plasmid with or without the hSTAT2 transgene construct, infected with PIV5 for 24 h prior to luciferase assay. Normalized to co-transfected Renilla luciferase. Bars indicate the mean normalized to percent of maximum (n=3) and error bars indicate standard deviation. (C) Ubiquitous STAT2 expression in transgenic mice. Protein extracts (20 μg) from isolated transgenic mice were subject to immunoblot to detect human STAT2. Ubiquitous expression of the transgene throughout the mouse was observed.

    Techniques Used: Expressing, Transgenic Assay, Ubiquitin Proteomics, Transfection, Luciferase, Plasmid Preparation, Construct, Infection, Standard Deviation, Isolation, Western Blot

    Fig. 3. Human STAT2 activity in mouse does not alter IFN signaling. (A) Splenocytes from transgenic and wild-type mice were treated with murine IFNβ for 5–30 min. Cells were immediately lysed and processed for immunoblot with antibodies that recognize human and mouse tyrosine phosphorylated STAT2, human STAT2, mouse STAT2 and STAT1α/β (recognizes human and mouse). (B) Splenocytes from transgenic and wild-type mice were treated with IFNβ for 10 min prior to lysate preparation and anti-STAT1 immunoprecipitation. Precipitated proteins were separated by SDS–PAGE and processed for immunoblot with antibodies for human STAT2, mouse STAT2 and tyrosine phosphorylated STAT2. (C) Splenocytes from hSTAT2 transgenic and wild-type mice were isolated and treated with mIFNβ for 6 or 18 h prior to RNA isolation and reverse transcription. Real-time PCR with primers specific for Mx1 and Ifi47 were performed and normalized to GAPDH. Graphs indicate average values for n=3, with error bars to represent standard deviation. (D) Transgenic and wild-type MEFs were pretreated 2 h with murine IFNβ, then infected with VSV (1 pfu/cell) for 16 h. Infectious virus released into the supernatant was estimated by titration on CV1 cells. IFN treatment provides a similar level of protection in transgenic and wild-type cells. Graph shows data from an individual VSV titration experiment. (E) Splenocytes were isolated from transgenic and wild-type mice and stimulated with IFNβ for 20′ prior to lysis. Whole cell extracts were separated by SDS–PAGE and immunoblotted with antibodies to detect STAT4 and tyrosine phosphorylated STAT4. IFNβ induces STAT4 activation in both wild-type and transgenic splenocytes.
    Figure Legend Snippet: Fig. 3. Human STAT2 activity in mouse does not alter IFN signaling. (A) Splenocytes from transgenic and wild-type mice were treated with murine IFNβ for 5–30 min. Cells were immediately lysed and processed for immunoblot with antibodies that recognize human and mouse tyrosine phosphorylated STAT2, human STAT2, mouse STAT2 and STAT1α/β (recognizes human and mouse). (B) Splenocytes from transgenic and wild-type mice were treated with IFNβ for 10 min prior to lysate preparation and anti-STAT1 immunoprecipitation. Precipitated proteins were separated by SDS–PAGE and processed for immunoblot with antibodies for human STAT2, mouse STAT2 and tyrosine phosphorylated STAT2. (C) Splenocytes from hSTAT2 transgenic and wild-type mice were isolated and treated with mIFNβ for 6 or 18 h prior to RNA isolation and reverse transcription. Real-time PCR with primers specific for Mx1 and Ifi47 were performed and normalized to GAPDH. Graphs indicate average values for n=3, with error bars to represent standard deviation. (D) Transgenic and wild-type MEFs were pretreated 2 h with murine IFNβ, then infected with VSV (1 pfu/cell) for 16 h. Infectious virus released into the supernatant was estimated by titration on CV1 cells. IFN treatment provides a similar level of protection in transgenic and wild-type cells. Graph shows data from an individual VSV titration experiment. (E) Splenocytes were isolated from transgenic and wild-type mice and stimulated with IFNβ for 20′ prior to lysis. Whole cell extracts were separated by SDS–PAGE and immunoblotted with antibodies to detect STAT4 and tyrosine phosphorylated STAT4. IFNβ induces STAT4 activation in both wild-type and transgenic splenocytes.

    Techniques Used: Activity Assay, Transgenic Assay, Western Blot, Immunoprecipitation, SDS Page, Isolation, Reverse Transcription, Real-time Polymerase Chain Reaction, Standard Deviation, Infection, Virus, Titration, Lysis, Activation Assay

    Fig. 5. Transgenic mouse cells support enhanced PIV5 replication. (A) MEFs were infected with PIV5 for 24 h before additional (1000 U/ml) exogenous IFNβ for another 24 h. Cells were lysed and processed for immunoblot with human STAT2 and P/Vantibodies. (B) PIV5 titer from MEFs infected at low MOI (1 pfu/cell) with PIV5 after 24 and 48 h. Viral supernatant was titered by serial dilution on CV-1 cells. Results show greater viral replication in transgenic MEFs.
    Figure Legend Snippet: Fig. 5. Transgenic mouse cells support enhanced PIV5 replication. (A) MEFs were infected with PIV5 for 24 h before additional (1000 U/ml) exogenous IFNβ for another 24 h. Cells were lysed and processed for immunoblot with human STAT2 and P/Vantibodies. (B) PIV5 titer from MEFs infected at low MOI (1 pfu/cell) with PIV5 after 24 and 48 h. Viral supernatant was titered by serial dilution on CV-1 cells. Results show greater viral replication in transgenic MEFs.

    Techniques Used: Transgenic Assay, Infection, Western Blot, Serial Dilution



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    phosphospecific antibodies against stat2  (Santa Cruz Biotechnology)
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    Santa Cruz Biotechnology phosphospecific antibodies against stat2
    Fig. 1. PIV5 V protein binds human but not mouse <t>STAT2.</t> Mouse NIH3T3 cells or human 2fTGH and HEK293Tcells were transfected with either FLAG-tagged PIV5 V protein (V) or FLAG vector control (C). Parallel samples of NIH3T3 were co-transfected with human STAT2 expression vector (hST2). Whole cell extracts were immunoprecipitated with FLAG-M2 agarose beads, eluted with SDS–PAGE buffer and processed for immunoblot with specific antibodies indicated.
    Phosphospecific Antibodies Against Stat2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphospecific antibodies against stat2/product/Santa Cruz Biotechnology
    Average 95 stars, based on 1 article reviews
    phosphospecific antibodies against stat2 - by Bioz Stars, 2026-02
    95/100 stars
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    Fig. 1. PIV5 V protein binds human but not mouse STAT2. Mouse NIH3T3 cells or human 2fTGH and HEK293Tcells were transfected with either FLAG-tagged PIV5 V protein (V) or FLAG vector control (C). Parallel samples of NIH3T3 were co-transfected with human STAT2 expression vector (hST2). Whole cell extracts were immunoprecipitated with FLAG-M2 agarose beads, eluted with SDS–PAGE buffer and processed for immunoblot with specific antibodies indicated.

    Journal: Virology

    Article Title: Enabled interferon signaling evasion in an immune-competent transgenic mouse model of parainfluenza virus 5 infection.

    doi: 10.1016/j.virol.2007.10.001

    Figure Lengend Snippet: Fig. 1. PIV5 V protein binds human but not mouse STAT2. Mouse NIH3T3 cells or human 2fTGH and HEK293Tcells were transfected with either FLAG-tagged PIV5 V protein (V) or FLAG vector control (C). Parallel samples of NIH3T3 were co-transfected with human STAT2 expression vector (hST2). Whole cell extracts were immunoprecipitated with FLAG-M2 agarose beads, eluted with SDS–PAGE buffer and processed for immunoblot with specific antibodies indicated.

    Article Snippet: Phosphospecific antibodies against STAT2 and STAT4 as well as antibodies against murine STAT4 and the unique C-term region of human STAT2 were obtained from Santa Cruz Biotechnology (Santa Cruz, CA) and used according to the manufacturer's instructions.

    Techniques: Transfection, Plasmid Preparation, Control, Expressing, Immunoprecipitation, SDS Page, Western Blot

    Fig. 2. Expression of human STAT2 in transgenic mice. (A) The 2555-bp open reading frame encoding human STAT2 was flanked by the human ubiquitin C promoter on the 5′ end and an SV40 splice site and poly A tail on the 3′ end. (B) NIH3T3 cells were transfected with the 5xISRE-luciferase plasmid with or without the hSTAT2 transgene construct, infected with PIV5 for 24 h prior to luciferase assay. Normalized to co-transfected Renilla luciferase. Bars indicate the mean normalized to percent of maximum (n=3) and error bars indicate standard deviation. (C) Ubiquitous STAT2 expression in transgenic mice. Protein extracts (20 μg) from isolated transgenic mice were subject to immunoblot to detect human STAT2. Ubiquitous expression of the transgene throughout the mouse was observed.

    Journal: Virology

    Article Title: Enabled interferon signaling evasion in an immune-competent transgenic mouse model of parainfluenza virus 5 infection.

    doi: 10.1016/j.virol.2007.10.001

    Figure Lengend Snippet: Fig. 2. Expression of human STAT2 in transgenic mice. (A) The 2555-bp open reading frame encoding human STAT2 was flanked by the human ubiquitin C promoter on the 5′ end and an SV40 splice site and poly A tail on the 3′ end. (B) NIH3T3 cells were transfected with the 5xISRE-luciferase plasmid with or without the hSTAT2 transgene construct, infected with PIV5 for 24 h prior to luciferase assay. Normalized to co-transfected Renilla luciferase. Bars indicate the mean normalized to percent of maximum (n=3) and error bars indicate standard deviation. (C) Ubiquitous STAT2 expression in transgenic mice. Protein extracts (20 μg) from isolated transgenic mice were subject to immunoblot to detect human STAT2. Ubiquitous expression of the transgene throughout the mouse was observed.

    Article Snippet: Phosphospecific antibodies against STAT2 and STAT4 as well as antibodies against murine STAT4 and the unique C-term region of human STAT2 were obtained from Santa Cruz Biotechnology (Santa Cruz, CA) and used according to the manufacturer's instructions.

    Techniques: Expressing, Transgenic Assay, Ubiquitin Proteomics, Transfection, Luciferase, Plasmid Preparation, Construct, Infection, Standard Deviation, Isolation, Western Blot

    Fig. 3. Human STAT2 activity in mouse does not alter IFN signaling. (A) Splenocytes from transgenic and wild-type mice were treated with murine IFNβ for 5–30 min. Cells were immediately lysed and processed for immunoblot with antibodies that recognize human and mouse tyrosine phosphorylated STAT2, human STAT2, mouse STAT2 and STAT1α/β (recognizes human and mouse). (B) Splenocytes from transgenic and wild-type mice were treated with IFNβ for 10 min prior to lysate preparation and anti-STAT1 immunoprecipitation. Precipitated proteins were separated by SDS–PAGE and processed for immunoblot with antibodies for human STAT2, mouse STAT2 and tyrosine phosphorylated STAT2. (C) Splenocytes from hSTAT2 transgenic and wild-type mice were isolated and treated with mIFNβ for 6 or 18 h prior to RNA isolation and reverse transcription. Real-time PCR with primers specific for Mx1 and Ifi47 were performed and normalized to GAPDH. Graphs indicate average values for n=3, with error bars to represent standard deviation. (D) Transgenic and wild-type MEFs were pretreated 2 h with murine IFNβ, then infected with VSV (1 pfu/cell) for 16 h. Infectious virus released into the supernatant was estimated by titration on CV1 cells. IFN treatment provides a similar level of protection in transgenic and wild-type cells. Graph shows data from an individual VSV titration experiment. (E) Splenocytes were isolated from transgenic and wild-type mice and stimulated with IFNβ for 20′ prior to lysis. Whole cell extracts were separated by SDS–PAGE and immunoblotted with antibodies to detect STAT4 and tyrosine phosphorylated STAT4. IFNβ induces STAT4 activation in both wild-type and transgenic splenocytes.

    Journal: Virology

    Article Title: Enabled interferon signaling evasion in an immune-competent transgenic mouse model of parainfluenza virus 5 infection.

    doi: 10.1016/j.virol.2007.10.001

    Figure Lengend Snippet: Fig. 3. Human STAT2 activity in mouse does not alter IFN signaling. (A) Splenocytes from transgenic and wild-type mice were treated with murine IFNβ for 5–30 min. Cells were immediately lysed and processed for immunoblot with antibodies that recognize human and mouse tyrosine phosphorylated STAT2, human STAT2, mouse STAT2 and STAT1α/β (recognizes human and mouse). (B) Splenocytes from transgenic and wild-type mice were treated with IFNβ for 10 min prior to lysate preparation and anti-STAT1 immunoprecipitation. Precipitated proteins were separated by SDS–PAGE and processed for immunoblot with antibodies for human STAT2, mouse STAT2 and tyrosine phosphorylated STAT2. (C) Splenocytes from hSTAT2 transgenic and wild-type mice were isolated and treated with mIFNβ for 6 or 18 h prior to RNA isolation and reverse transcription. Real-time PCR with primers specific for Mx1 and Ifi47 were performed and normalized to GAPDH. Graphs indicate average values for n=3, with error bars to represent standard deviation. (D) Transgenic and wild-type MEFs were pretreated 2 h with murine IFNβ, then infected with VSV (1 pfu/cell) for 16 h. Infectious virus released into the supernatant was estimated by titration on CV1 cells. IFN treatment provides a similar level of protection in transgenic and wild-type cells. Graph shows data from an individual VSV titration experiment. (E) Splenocytes were isolated from transgenic and wild-type mice and stimulated with IFNβ for 20′ prior to lysis. Whole cell extracts were separated by SDS–PAGE and immunoblotted with antibodies to detect STAT4 and tyrosine phosphorylated STAT4. IFNβ induces STAT4 activation in both wild-type and transgenic splenocytes.

    Article Snippet: Phosphospecific antibodies against STAT2 and STAT4 as well as antibodies against murine STAT4 and the unique C-term region of human STAT2 were obtained from Santa Cruz Biotechnology (Santa Cruz, CA) and used according to the manufacturer's instructions.

    Techniques: Activity Assay, Transgenic Assay, Western Blot, Immunoprecipitation, SDS Page, Isolation, Reverse Transcription, Real-time Polymerase Chain Reaction, Standard Deviation, Infection, Virus, Titration, Lysis, Activation Assay

    Fig. 5. Transgenic mouse cells support enhanced PIV5 replication. (A) MEFs were infected with PIV5 for 24 h before additional (1000 U/ml) exogenous IFNβ for another 24 h. Cells were lysed and processed for immunoblot with human STAT2 and P/Vantibodies. (B) PIV5 titer from MEFs infected at low MOI (1 pfu/cell) with PIV5 after 24 and 48 h. Viral supernatant was titered by serial dilution on CV-1 cells. Results show greater viral replication in transgenic MEFs.

    Journal: Virology

    Article Title: Enabled interferon signaling evasion in an immune-competent transgenic mouse model of parainfluenza virus 5 infection.

    doi: 10.1016/j.virol.2007.10.001

    Figure Lengend Snippet: Fig. 5. Transgenic mouse cells support enhanced PIV5 replication. (A) MEFs were infected with PIV5 for 24 h before additional (1000 U/ml) exogenous IFNβ for another 24 h. Cells were lysed and processed for immunoblot with human STAT2 and P/Vantibodies. (B) PIV5 titer from MEFs infected at low MOI (1 pfu/cell) with PIV5 after 24 and 48 h. Viral supernatant was titered by serial dilution on CV-1 cells. Results show greater viral replication in transgenic MEFs.

    Article Snippet: Phosphospecific antibodies against STAT2 and STAT4 as well as antibodies against murine STAT4 and the unique C-term region of human STAT2 were obtained from Santa Cruz Biotechnology (Santa Cruz, CA) and used according to the manufacturer's instructions.

    Techniques: Transgenic Assay, Infection, Western Blot, Serial Dilution